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testes  (TaKaRa)


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    Structured Review

    TaKaRa testes
    Testes, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/testis+total+rna/Human+Testis+Total+RNA/bio_rxiv__64898__2026__02__14__705912-72-4-8
    Average 94 stars, based on 248 article reviews
    testes - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Cloning:

    Article Title: Expression characteristics of the SUMOylation genes SUMO-1 and Ubc9 in the developing testis and ovary of Chinese mitten crab, Eriocheir sinensis.
    Article Snippet: Abbreviations: Cdc2, cyclin-dependent kinase2; E1 enzyme; E2s, (SUMO) ubiquitin-conjugating enzymes; EsSUMO-1, Eriocheir sinensis small ubiquitin-like modifi SUMO-conjugating enzyme; EST, expressed sequence t NBT/BCIP, Nitro blue tetrazolium chloride/5-Bromo-4-c luidine salt; ORF, open reading frame; RACE, Rapid Am GAP1, Ran GTPase-activating protein 1; SIM, SUMOsodium citrate: SUMO-1 (SMT3), small ubiquitin-like ubiquitin-like modifier 2/3; UBC, ubiquitin-conjugating; Ulp1 (SENPs), SUMO-specific proteases.. ⁎ Corresponding author at: School of Life Science, E North Zhong-Shan Road 3663, Shanghai, China.. Tel.

    Reverse Transcription:

    Article Title: Expression characteristics of the SUMOylation genes SUMO-1 and Ubc9 in the developing testis and ovary of Chinese mitten crab, Eriocheir sinensis.
    Article Snippet: Abbreviations: Cdc2, cyclin-dependent kinase2; E1 enzyme; E2s, (SUMO) ubiquitin-conjugating enzymes; EsSUMO-1, Eriocheir sinensis small ubiquitin-like modifi SUMO-conjugating enzyme; EST, expressed sequence t NBT/BCIP, Nitro blue tetrazolium chloride/5-Bromo-4-c luidine salt; ORF, open reading frame; RACE, Rapid Am GAP1, Ran GTPase-activating protein 1; SIM, SUMOsodium citrate: SUMO-1 (SMT3), small ubiquitin-like ubiquitin-like modifier 2/3; UBC, ubiquitin-conjugating; Ulp1 (SENPs), SUMO-specific proteases.. ⁎ Corresponding author at: School of Life Science, E North Zhong-Shan Road 3663, Shanghai, China.. Tel.

    Article Title: Structured RNAs in the ENCODE selected regions of the human genome
    Article Snippet: .. Brain, heart, kidney, liver, lung, and testis total RNA (0.1 μg each) was mixed and reversed-transcribed in 25 μL with AMV Reverse Transcriptase XL in the presence of dNTP nucleotide analogs to avoid amplification of genomic DNA contaminants; RNase Inhibitor; and MgCl 2 (mRNA Selective PCR kit; Takara). ..

    Clone Assay:

    Article Title: Involvement of Transforming Growth Factor Beta Family Genes in Gonadal Differentiation in Japanese Eel, Anguilla japonica, According to Sex-Related Gene Expressions
    Article Snippet: .. The amh, amhr2 , and gsdf cDNAs were cloned from the testis total RNA of Japanese eels by RT-PCR and rapid amplification of cDNA ends (RACE), following the manufacturer’s protocol (SMARTer TM RACE cDNA Amplification Kit, Clontech, Tokyo, Japan). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Involvement of Transforming Growth Factor Beta Family Genes in Gonadal Differentiation in Japanese Eel, Anguilla japonica, According to Sex-Related Gene Expressions
    Article Snippet: .. The amh, amhr2 , and gsdf cDNAs were cloned from the testis total RNA of Japanese eels by RT-PCR and rapid amplification of cDNA ends (RACE), following the manufacturer’s protocol (SMARTer TM RACE cDNA Amplification Kit, Clontech, Tokyo, Japan). ..

    Rapid Amplification of cDNA Ends:

    Article Title: Involvement of Transforming Growth Factor Beta Family Genes in Gonadal Differentiation in Japanese Eel, Anguilla japonica, According to Sex-Related Gene Expressions
    Article Snippet: .. The amh, amhr2 , and gsdf cDNAs were cloned from the testis total RNA of Japanese eels by RT-PCR and rapid amplification of cDNA ends (RACE), following the manufacturer’s protocol (SMARTer TM RACE cDNA Amplification Kit, Clontech, Tokyo, Japan). ..

    Amplification:

    Article Title: Involvement of Transforming Growth Factor Beta Family Genes in Gonadal Differentiation in Japanese Eel, Anguilla japonica, According to Sex-Related Gene Expressions
    Article Snippet: .. The amh, amhr2 , and gsdf cDNAs were cloned from the testis total RNA of Japanese eels by RT-PCR and rapid amplification of cDNA ends (RACE), following the manufacturer’s protocol (SMARTer TM RACE cDNA Amplification Kit, Clontech, Tokyo, Japan). ..

    Article Title: Molecular cloning, expression analysis and transcript localization of testicular orphan nuclear receptor 2 in the male catfish, Clarias batrachus.
    Article Snippet: Testicular receptor 2 (TR2; also known as Nr2c1) is one of the first orphan nuclear receptors identified and known to regulate various physiological process with or without any ligand.. In this study, we report the cloning of full length nr2c1 and its expression analysis during gonadal development, seasonal testicular cycle and after human chorionic gonadotropin (hCG) induction.. In addition, in situ hybridization (ISH) was performed to localize nr2c1 transcripts in adult testis and whole catfish (1 day post hatch).

    Article Title: Identification of multiple dmrt1s in catfish: localization, dimorphic expression pattern, changes during testicular cycle and after methyltestosterone treatment
    Article Snippet: .. SMART 5 0 and 3 0 cDNA templates were made from testis total RNA according to manufacturer’s protocol using the SMART RACE cDNA amplification kit (Clontech, Tokyo, Japan). ..

    Article Title: Structured RNAs in the ENCODE selected regions of the human genome
    Article Snippet: .. Brain, heart, kidney, liver, lung, and testis total RNA (0.1 μg each) was mixed and reversed-transcribed in 25 μL with AMV Reverse Transcriptase XL in the presence of dNTP nucleotide analogs to avoid amplification of genomic DNA contaminants; RNase Inhibitor; and MgCl 2 (mRNA Selective PCR kit; Takara). ..

    Synthesized:

    Article Title: Molecular cloning, expression analysis and transcript localization of testicular orphan nuclear receptor 2 in the male catfish, Clarias batrachus.
    Article Snippet: Testicular receptor 2 (TR2; also known as Nr2c1) is one of the first orphan nuclear receptors identified and known to regulate various physiological process with or without any ligand.. In this study, we report the cloning of full length nr2c1 and its expression analysis during gonadal development, seasonal testicular cycle and after human chorionic gonadotropin (hCG) induction.. In addition, in situ hybridization (ISH) was performed to localize nr2c1 transcripts in adult testis and whole catfish (1 day post hatch).

    Article Title: Hdh-Tektin-4 Regulates Motility of Fresh and Cryopreserved Sperm in Pacific Abalone, Haliotis discus hannai
    Article Snippet: First-strand cDNAs were synthesized from 1∼4 μl of total RNAs using an oligo(dT) primer (Sigma) and superscript III First-strand cDNA synthesis kit (Invitrogen, USA). .. Using a SMARTer® RACE 5′/3′ Kit (Takara Bio Inc., Japan), 5′- and 3′-RACE cDNAs were synthesized from 1 μl of testis total RNA. ..

    Isolation:

    Article Title: Molecular cloning of two estrogen receptors expressed in the testis of the Japanese common goby, Acanthogobius flavimanus.
    Article Snippet: BioOne sees sustainable scholarly publishing as an inherently collaborative enterprise connecting authors, nonprofit publishers, academic institutions, research libraries, and research funders in the common goal of maximizing access to critical research.. Molecular Cloning of Two Estrogen Receptors Expressed in the Testis of the Japanese Common Goby, Acanthogobius flavimanus Author(s): Katsutoshi Ito, Kazuhiko Mochida, Kazunori Fujii Source: Zoological Science, 24(10):986-996.. 2007.

    Polymerase Chain Reaction:

    Article Title: Structured RNAs in the ENCODE selected regions of the human genome
    Article Snippet: .. Brain, heart, kidney, liver, lung, and testis total RNA (0.1 μg each) was mixed and reversed-transcribed in 25 μL with AMV Reverse Transcriptase XL in the presence of dNTP nucleotide analogs to avoid amplification of genomic DNA contaminants; RNase Inhibitor; and MgCl 2 (mRNA Selective PCR kit; Takara). ..



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    The different study groups and main steps of the study design. Control, male gonadal tissue from men with normal <t>spermatogenesis;</t> hTPC, human testicular primary cells; NC, negative control with GFP (green fluorescent protein) expression; NOA, non-obstructive azoospermia; negative control NOA, patient with NOA and no mutation in the TKTL1 gene; NOA patient with mutation in the TKTL1 , patient with NOA with mutation in the TKTL1 gene; TKTL1, cells with the overexpressed TKTL1 gene; WT, wild type cells in in-vitro culture medium only.
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    Image Search Results


    The different study groups and main steps of the study design. Control, male gonadal tissue from men with normal spermatogenesis; hTPC, human testicular primary cells; NC, negative control with GFP (green fluorescent protein) expression; NOA, non-obstructive azoospermia; negative control NOA, patient with NOA and no mutation in the TKTL1 gene; NOA patient with mutation in the TKTL1 , patient with NOA with mutation in the TKTL1 gene; TKTL1, cells with the overexpressed TKTL1 gene; WT, wild type cells in in-vitro culture medium only.

    Journal: Reproductive biomedicine online

    Article Title: TKTL1: a new candidate gene in non-obstructive azoospermia

    doi: 10.1016/j.rbmo.2025.104895

    Figure Lengend Snippet: The different study groups and main steps of the study design. Control, male gonadal tissue from men with normal spermatogenesis; hTPC, human testicular primary cells; NC, negative control with GFP (green fluorescent protein) expression; NOA, non-obstructive azoospermia; negative control NOA, patient with NOA and no mutation in the TKTL1 gene; NOA patient with mutation in the TKTL1 , patient with NOA with mutation in the TKTL1 gene; TKTL1, cells with the overexpressed TKTL1 gene; WT, wild type cells in in-vitro culture medium only.

    Article Snippet: To characterize the hTP cells ( n = 3 experimental replicates), the commercial total RNA obtained from normal testicular tissues with normal spermatogenesis was used ( n = 3) (catalogue number 636533) (Clontech Laboratories, Mountain View, CA, USA); (catalogue number 540049) (Agilent Life Technologies, Carlsbad, CA, USA) (catalogue number AM7972) (Ambion Life Technologies, Carlsbad, CA, USA) and also TCam-2 cells ( n = 3 experimental replicates) (the testicular seminoma cell line), which were gifted by Dr Sohei Kitazawa (Ehime University Graduate School of Medicine, Japan) and described in detail by .

    Techniques: Control, Negative Control, Expressing, Mutagenesis, In Vitro

    Verification of selected genes by real-time polymerase chain reaction ( CCN2, CSF3, FSD1L, HERC5, HES1, HSPA1B, PTBP2, SMOC1 ) in testicular tissue from patients with non-obstructive azoospermia (NOA) and controls. Control, male gonadal tissue from men with normal spermatogenesis ( n = 2 technical repeats); NOA TKTL1 , patient with NOA without mutation in the TKTL1 gene ( n = 2 technical repeats); NOA TKTL1 mut , patient with NOA with mutation in the TKTL1 gene ( n = 2 technical repeats). Data presented as mean + SD. P -values are presented on each graph. Data analysed by one-way analysis of variance with Tukey’s multiple comparisons test.

    Journal: Reproductive biomedicine online

    Article Title: TKTL1: a new candidate gene in non-obstructive azoospermia

    doi: 10.1016/j.rbmo.2025.104895

    Figure Lengend Snippet: Verification of selected genes by real-time polymerase chain reaction ( CCN2, CSF3, FSD1L, HERC5, HES1, HSPA1B, PTBP2, SMOC1 ) in testicular tissue from patients with non-obstructive azoospermia (NOA) and controls. Control, male gonadal tissue from men with normal spermatogenesis ( n = 2 technical repeats); NOA TKTL1 , patient with NOA without mutation in the TKTL1 gene ( n = 2 technical repeats); NOA TKTL1 mut , patient with NOA with mutation in the TKTL1 gene ( n = 2 technical repeats). Data presented as mean + SD. P -values are presented on each graph. Data analysed by one-way analysis of variance with Tukey’s multiple comparisons test.

    Article Snippet: To characterize the hTP cells ( n = 3 experimental replicates), the commercial total RNA obtained from normal testicular tissues with normal spermatogenesis was used ( n = 3) (catalogue number 636533) (Clontech Laboratories, Mountain View, CA, USA); (catalogue number 540049) (Agilent Life Technologies, Carlsbad, CA, USA) (catalogue number AM7972) (Ambion Life Technologies, Carlsbad, CA, USA) and also TCam-2 cells ( n = 3 experimental replicates) (the testicular seminoma cell line), which were gifted by Dr Sohei Kitazawa (Ehime University Graduate School of Medicine, Japan) and described in detail by .

    Techniques: Real-time Polymerase Chain Reaction, Control, Mutagenesis